Coding

Part:BBa_K4812022:Design

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-12)


proS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 460
    Illegal EcoRI site found at 625
    Illegal PstI site found at 508
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 460
    Illegal EcoRI site found at 625
    Illegal PstI site found at 508
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 460
    Illegal EcoRI site found at 625
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 460
    Illegal EcoRI site found at 625
    Illegal PstI site found at 508
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 460
    Illegal EcoRI site found at 625
    Illegal PstI site found at 508
    Illegal AgeI site found at 595
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Inclusion of endogenous proS promoter may be necessary for gene code expansion applications.


Source

E. Coli K12 MG1655

References